The Complementary Effects of 2-mercaptoethanol and Trypsin in the Lysis of Hela Cells

Considerable interest has been expressed in recent years in the relative effects of different methods of DNA isolation on the physical, chemical, and biological properties of the material isolated. In particular, interest has been centered on methods of disrupting cells. Of these methods, mechanical abrasion, sonication, and chemical lysis have been most often reported. The effectiveness of mechanical methods in producing intact DNA is in doubt, inasmuch as DNA molecules